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The solution isnt guesswork

Several theories explain why headaches occur

Small-interfering RNA transfection Transfections of siRNAs (a final concentration of 40 nM, in the case of double depletion 20 nM each) were done using Lipofectamine RNAiMAX (Thermo Fisher Scientific, 13778150) according to the instructions of manufacturer (reverse transfection protocol)